Overview

  • Product name
    Donkey Anti-Goat IgG H&L (Biotin)
    See all IgG secondary antibodies
  • Target species
    Goat
  • Tested applications
    Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Immunogen

    Full length native Goat IgG (purified).

  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    Use donkey anti-goat IgG (H&L) biotin to confidently detect primary antibodies raised in goat in your immunohistochemistry, western blot, ELISA or immunoprecipitation. Get dilutions to get the best results in your IHC, WB, IP, or ELISA with donkey anti-goat IgG (H&L) biotin below.  

     

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
Dot blot Use at an assay dependent dilution.
ELISA 1/180000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
WB 1/2000 - 1/10000.

Images

  • ab6884 used to stain samples of whole heads of embryonic day 15 (E15) mice by Immunohistochemistry (Formalin-fixed, paraffin-embedded sections).
    Comparison of four staining methods. The heads were flash-frozen directly in isopentane on dry-ice. Once hardened, they were embedded in O.C.T compound. Frozen sections were postfixed in 4% paraformaldehyde in PBS for 20 minutes and quenched in 1.5% hydrogen peroxide for 30 minutes. Sections were then blocked for 2 hours at room temperature with 5% donkey serum in Tris-buffered saline with 0.1% Triton-X100 and then incubated with a goat anti-Nurr1 primary antibody 1/200 in 1% donkey serum in TBS at 4°C overnight. Biotinylated donkey anti-goat (ab6884) 1/500 in 1% donkey serum in TBS was applied for 2 hours at room temperature and reacted with avidin-biotinylated enzyme complex (ABC) using the Vectastain Elite kit and diaminobenzidene (DAB) according to the manufacturer's instructions. Images were taken directly on the PALM Microbeam to demonstrate the staining and image quality available to identify the different cell populations.

    Image a: Hematoxylin.
    Image b: Hematoxylin and Eosin.
    Image c: 0.1% cresyl violet in H2O.
    Image d: 1% cresyl violet in H2O.
    For hematoxylin and H&E staining (a, b), sections were rinsed with 70% EtOH and H2O, stained with Mayer's hematoxylin for 15 seconds, rinsed with H2O and 70% EtOH and stained with Accustain Eosin Y for 10 seconds (for H&E only). For cresyl violet staining (c, d), sections were rehydrated in decreasing concentrations of ethanol, stained in cresyl violet for 45 seconds and dehydrated in increasing concentrations of ethanol. 1% cresyl violet (d) provided the best morphological details and allowed clear distinction between cortical plate (CP) and subplate (SP).

References

This product has been referenced in:

See all 10 Publications for this product

Customer reviews and Q&As

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The Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Biotin) (ab6884) is biotin conjugated. This antibody has been shown to work successfully in Western blotting and ca...

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These product are both available from our Abcam US office located in Cambridge, MA. We even offer same day shipping in Boston, MA area.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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