Donkey Anti-Goat IgG H&L (Biotin) preadsorbed (ab7124)

Overview

  • Product nameDonkey Anti-Goat IgG H&L (Biotin) preadsorbed
    See all IgG secondary antibodies
  • Target speciesGoat
  • Tested applicationsSuitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Minimal
    cross-reactivity

    Chicken, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details
  • Immunogen

    Full length native Goat IgG (purified).

  • ConjugationBiotin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
  • ClonalityPolyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
WB
  • Application notesDot: Use at an assay dependant dilution.
    ELISA: 1/16000 - 1/64000.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    IM: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Donkey Anti-Goat IgG H&L (Biotin) preadsorbed (ab7124)

    This product has been referenced in:
    • Rienstra H  et al. Donor and recipient origin of mesenchymal and endothelial cells in chronic renal allograft remodeling. Am J Transplant 9:463-72 (2009). IHC-P . Read more (PubMed: 19260830) »
    • Broekema M  et al. Bone marrow-derived myofibroblasts contribute to the renal interstitial myofibroblast population and produce procollagen I after ischemia/reperfusion in rats. J Am Soc Nephrol 18:165-75 (2007). Read more (PubMed: 17135399) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your message and for providing this further information.

    Reviewing the details, I agree there seems to be some crossreactivity to mouse IgG. I can confirm that this antibody has not specifically been pre absorbedwith mouse, so ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"