Donkey Anti-Goat IgG H&L (Biotin) preadsorbed (ab97113)

Overview

  • Product nameDonkey Anti-Goat IgG H&L (Biotin) preadsorbed
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Biotin), pre-adsorbed
  • Target speciesGoat
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, ELISA, WBmore details
  • Minimal
    cross-reactivity

    Chicken, Human, Mouse, Pig, Rabbit, Rat more details
  • ConjugationBiotin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
  • Conjugation notesBiotin/ antibody protein ratio is 4:1
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/2500.
ICC/IF 1/100 - 1/500.
ELISA 1/10000 - 1/200000. (as Primary).
WB 1/10000 - 1/200000. (Chemiluminescent).

References for Donkey Anti-Goat IgG H&L (Biotin) preadsorbed (ab97113)

ab97113 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"