Overview

  • Product nameDonkey Anti-Goat IgG H&L (Cy3.5 ®) preadsorbedSee all IgG secondary antibodies ...
  • Description
    Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy3.5 ®), pre-adsorbed
  • Target speciesGoat
  • Tested applicationsICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISAmore details
  • Minimal
    cross-reactivity

    Cow, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details
  • Immunogen

    Full length native Goat IgG (purified).

  • ConjugationCy3.5 ®. Ex: 581nm, Em: 596nm

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
  • Conjugation notesCy3.5 (Cyanine 3.5-OSu) (Molecular Weight 1286 daltons) Absorption Wavelength: 581 nm Emission Wavelength: 596 nm Fluorochrome/Protein Ratio: 6.9 moles Cy3.5 per mole of Donkey IgG
  • Clonality Polyclonal
  • General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
IHC-Fr
ICC
Flow Cyt
ELISA
  • Application notesFlow Cyt: Use at an assay dependant concentration.
    ICC: Use at an assay dependant concentration.
    IF: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Donkey Anti-Goat IgG H&L (Cy3.5 ®) preadsorbed (ab6950)

    This product has been referenced in:
    • Heydendael W  et al. Optogenetic examination identifies a context-specific role for orexins/hypocretins in anxiety-related behavior. Physiol Behav N/A:N/A (2013). Read more (PubMed: 24140988) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab6950.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"