Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566)

Overview

  • Product nameDonkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy5 ®), pre-adsorbed
  • Target speciesGoat
  • Tested applicationsICC, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, WB, ELISAmore details
  • Minimal
    cross-reactivity

    Chicken, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details
  • Immunogen

    Goat IgG, whole molecule

  • ConjugationCy5 ®. Ex: 650nm, Em: 667nm

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferpH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.
  • Conjugation notesCy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 6.0 moles Cy5 per mole of Goat IgG
  • Clonality Polyclonal
  • General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondaryantibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent dilution.
ELISA 1/10000 - 1/50000.

Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed images

  • Lane 1 :
    Lanes 2 - 3 : Goat anti-UCP1 at 1/200 dilution

    Lane 1 : Marker
    Lane 2 : Mouse brown adipose tissue lysate at 70 µg
    Lane 3 : Mouse brown adipose tissue lysate at 70 µg

    Secondary
    Lanes 2 - 3 : Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566) at 1/2500 dilution


    Observed band size : 33 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous Abreview

References for Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566)

This product has been referenced in:
  • Alfieri A  et al. Sulforaphane preconditioning of the Nrf2/HO-1 defense pathway protects the cerebral vasculature against blood-brain barrier disruption and neurological deficits in stroke. Free Radic Biol Med 65:1012-22 (2013). Read more (PubMed: 24017972) »
  • Kainov DE  et al. Differential Effects of NS1 Proteins of Human Pandemic H1N1/2009, Avian Highly Pathogenic H5N1, and Low Pathogenic H5N2 Influenza A Viruses on Cellular Pre-mRNA Polyadenylation and mRNA Translation. J Biol Chem 286:7239-47 (2011). WB . Read more (PubMed: 21163951) »

See all 5 Publications for this product

Product Wall

Application Western blot
All lanes: Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566) 1:2500
Lane 1: Marker
Lane 2: Protein lysate of BAT (Mouse 1), Protein 70µg
Lane 3: Protein lysate of BAT (Mouse 2), Protein 70µg
Lanes 2+3: Primary Antibody anti-UCP1 1:200
Predicted band size 33kDa
Username

Abcam user community

Verified customer

Submitted Aug 20 2014

Thank you for your enquiry. I believe Cy5.29 OSu is the same as Cy5, but refers more specifically to the cyanine derivative, a succinimide ester, that is used for conjugation. The following paper describes this ester: Cytometry. 1990;11(3):418-3...

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This antibody will indeed recognise the other secondary. You need to use a secondary antibody to the mouse IgG raised in a species other than goat, preferably donkey (if you want to use ab6566). Look for a donkey anti-mouse IgG fluorphore conjugated se...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"