Donkey Anti-Goat IgG H&L (Cy5.5 ®) preadsorbed (ab6951)

Overview

  • Product name
    Donkey Anti-Goat IgG H&L (Cy5.5 ®) preadsorbed
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy5.5 ®), pre-adsorbed
  • Target species
    Goat
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISAmore details
  • Minimal
    cross-reactivity

    Cow, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details
  • Immunogen

    Full length native Goat IgG (purified).

  • Conjugation
    Cy5.5 ®. Ex: 678nm, Em: 703nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
  • Conjugation notes
    Cy5.5 (Cyanine 5.5-OSu) (Molecular Weight 1312 daltons) Absorption Wavelength: 678 nm Emission Wavelength: 703 nm Fluorochrome/Protein Ratio: 4.1 moles Cy5.5 per mole of Donkey IgG
  • Clonality
    Polyclonal
  • General notes
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ELISA 1/10000 - 1/50000.

References for Donkey Anti-Goat IgG H&L (Cy5.5 ®) preadsorbed (ab6951)

This product has been referenced in:
  • Wilhelm T  et al. Spontaneous slow replication fork progression elicits mitosis alterations in homologous recombination-deficient mammalian cells. Proc Natl Acad Sci U S A 111:763-8 (2014). Read more (PubMed: 24347643) »
  • Miotto B  et al. The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability. Cell Rep 7:575-87 (2014). ICC/IF . Read more (PubMed: 24726359) »

See all 4 Publications for this product

Product Wall

Unfortunately we do not have data on the stability of C5.5 dyes on fixed stored IHC slides. This would depend on how they were imaged and the mounting medium that was used, as well as the exposure of the slides to light, however.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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