Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed (ab96935)

Overview

  • Product nameDonkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Goat IgG - H&L (DyLight® 488), pre-adsorbed
  • Target speciesGoat
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.
  • Tested applicationsSuitable for: Flow Cyt, IHC-P, ICC/IFmore details
  • Minimal
    cross-reactivity

    Chicken, Horse, Human, Mouse, Pig, Rabbit, Rat more details
  • ConjugationDyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.

Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed images

  • Immunohistochemical analysis of Paraformaldehyde perfusion-fixed frozen human neuroblastoma xenograft into a maurine host. The image is labelling LIMPII with ab87637 at 20°C for 1 hour at a dilution of 1/50 with 5% Donkey Serum. Permeabilization was done with 0.2% Triton. Secondary used was ab96935 at a 1/250 dilution.

    See Abreview

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed (ab96935)

ab96935 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Rat brains were fixed with paraformaldehyde 4%. Frozen section were realised (30µm).
Tissue sections were blocked with serum goat 5% + BSA 1% + Triton 0.2% during 2h.
First antibody was applied 1h (Iba-1 ref ab 5076) diluted 1/500, sections were washed twice with PBS.
Second antibody (ab 96935) was applied 1h , diluted 1/250, sections were washed again 3 time, before mounting with appropried medium.
Username

Ms. Francoise Geffroy

Verified customer

Submitted Aug 22 2014

Thank you for your message.
Yes, your customer can get a discount code for writing an Abreview about this secondary antibody. However, as always, we would need their email address and institute for our records.
I am looking forward to hearing...

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Thank you for contacting us.
You are right, this shouldn't be a problem in this case, as the "target protein" is not been altered due to fixation etc. In addition, the antibody does work in tissue already, therefore, it should work.
I hope th...

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Thank you for contacting Abcam. I am sorry that the product you received was not of the full volume. I have processed a free of charge replacement for you. You should be receiving information from your supplier shortly. Please feel free to contact me...

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Thank you very much for contacting us. Indeed, we assume that ab96935 should work with frozen sections. Having spoken with our Abreview department, I am happy to let you know that the customer is eligible for our testing discount promotion: Similar to ...

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Thank you for contacting us. I am sorry for the trouble caused here. This product is shipped in a ready-to-use form so reconstitution is unneccessary. Since you have not received the entire amount, 1ml, I would like to send you a free of charge replac...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"