Our Abpromise guarantee covers the use of ab96935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/50 - 1/200.|
|IHC-P||1/50 - 1/500.|
|ICC/IF||1/50 - 1/500.|
Immunohistochemical analysis of Paraformaldehyde perfusion-fixed frozen human neuroblastoma xenograft into a maurine host. The image is labelling LIMPII with ab87637 at 20°C for 1 hour at a dilution of 1/50 with 5% Donkey Serum. Permeabilization was done with 0.2% Triton. Secondary used was ab96935 at a 1/250 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"