Donkey Anti-Goat IgG H&L (FITC) preadsorbed (ab7121)

Overview

  • Product nameDonkey Anti-Goat IgG H&L (FITC) preadsorbed
    See all IgG secondary antibodies
  • Target speciesGoat
  • Tested applicationsSuitable for: ICC/IF, Immunomicroscopy, Flow Cyt, ELISA, IHC-P, IHC-Frmore details
  • Minimal
    cross-reactivity

    Chicken, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details
  • Immunogen

    Full length native Goat IgG (purified).

  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesDiaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles DTAF per mole of Rabbit IgG
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Immunomicroscopy
Flow Cyt
ELISA
IHC-P
IHC-Fr
  • Application notesFlow Cyt: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    IM: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Donkey Anti-Goat IgG H&L (FITC) preadsorbed (ab7121)

    This product has been referenced in:
    • Miranda E  et al. The intracellular accumulation of polymeric neuroserpin explains the severity of the dementia FENIB. Hum Mol Genet 17:1527-39 (2008). Read more (PubMed: 18267959) »
    • Grimsey N  et al. Temporal and spatial regulation of the phosphatidate phosphatases lipin 1 and 2. J Biol Chem 283:29166-74 (2008). Read more (PubMed: 18694939) »

    See all 3 Publications for this product

    Product Wall

    FITC-labelled antibodies are stable for at least 6 months at room temperature, although there may be a slight decrease in activity after 3 months. Storage at +4oC, will increase the shelf-life.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"