Overview

  • Product nameDonkey Anti-Goat IgG H&L (HRP)See all IgG secondary antibodies ...
  • Target speciesGoat
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applicationsIHC-P, ELISA, WB, ICCmore details
  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.1% Proclin
    Constituent: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notesMolar enzyme/ antibody protein ratio is 4:1
  • Clonality Polyclonal
  • IsotypeIgG
  • General notesPart of the AbExcel range.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000. Chemiluminescent: 1/1000 - 1/30000. In some cases the antibody may be diluted further than indicated.
ICC 1/200 - 1/500.

Donkey Anti-Goat IgG H&L (HRP) images

  • IHC image of beta actin staining in Human normal Colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8229, 10µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97110, 1/100 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-beta Actin antibody (ab8229) at 1 µg/ml and HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg per lane.

    Secondary
    Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97110) at 1/5000 dilution
    Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97110) at 1/10000 dilution
    Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97110) at 1/20000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 minutes

References for Donkey Anti-Goat IgG H&L (HRP) (ab97110)

This product has been referenced in:

See 1 Publication for this product

Product Wall

We have studied the effect of freeze thaw cycles on conjugated antibodies and have seen that they can have significant effect on HRP secondaries. As such we do not recommend stroage at -20C. These products should have good activity for one year or m...

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Thank you for your enquiry. Please find enclosed as requested the CoC for ab97110, lot number GR13665-10. I hope this information is helpful to you. Please do not hesitate to contact us if you need any additional information or advice.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"