• Product nameDonkey Anti-Goat IgG H&L (HRP)
    See all IgG secondary antibodies
  • Target speciesGoat
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applicationsSuitable for: IHC-P, ELISA, WB, ICCmore details
  • ConjugationHRP


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Proclin
    Constituent: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notesMolar enzyme/ antibody protein ratio is 4:1
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notesPart of the AbExcel range.
  • Research areas


Our Abpromise guarantee covers the use of ab97110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000. Chemiluminescent: 1/1000 - 1/30000. In some cases the antibody may be diluted further than indicated.
ICC 1/200 - 1/500.

Donkey Anti-Goat IgG H&L (HRP) images

  • IHC image of beta actin staining in Human normal Colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8229, 10µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97110, 1/100 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/200 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-beta Actin antibody (ab8229) at 1 µg/ml and HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg per lane.

    Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97110) at 1/5000 dilution
    Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97110) at 1/10000 dilution
    Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97110) at 1/20000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 minutes

References for Donkey Anti-Goat IgG H&L (HRP) (ab97110)

This product has been referenced in:
  • Yang LX  et al. Ginsenoside Rd Attenuates DNA Damage by Increasing Expression of DNA Glycosylase Endonuclease VIII-like Proteins after Focal Cerebral Ischemia. Chin Med J (Engl) 129:1955-62 (2016). WB . Read more (PubMed: 27503022) »
  • Wang X  et al. The Prostaglandin E2-EP3 Receptor Axis Regulates Anaplasma phagocytophilum-Mediated NLRC4 Inflammasome Activation. PLoS Pathog 12:e1005803 (2016). WB . Read more (PubMed: 27482714) »

See all 3 Publications for this product

Product Wall

Application Western blot
A excellent 2nd Ab for WB (1:2,000 dilution) used with ab166930 (1:2000).

Mr. Baptiste Jude

Verified customer

Submitted Jul 06 2016

Application Western blot
This antibody is good for western blot assay, a dilution of 1 to 5,000 could always offer a good detection signal, which was comparable to the dilution of 1 to 2,000.

Abcam user community

Verified customer

Submitted Nov 05 2015

Application Western blot
a good 2nd Ab for WB (1:5,000 dilution).

Abcam user community

Verified customer

Submitted Nov 02 2015

We have studied the effect of freeze thaw cycles on conjugated antibodies and have seen that they can have significant effect on HRP secondaries. As such we do not recommend stroage at -20C. These products should have good activity for one year or m...

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Thank you for your enquiry. Please find enclosed as requested the CoC for ab97110, lot number GR13665-10. I hope this information is helpful to you. Please do not hesitate to contact us if you need any additional information or advice.