Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125)

Overview

  • Product nameDonkey Anti-Goat IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Target speciesGoat
  • Tested applicationsSuitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IFmore details
  • Minimal
    cross-reactivity

    Chicken, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details
  • Immunogen

    Full length native Goat IgG (purified).

  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesHorseradish Peroxidase (HRP)
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent dilution.
ELISA 1/170000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
WB 1/2000 - 1/10000.
ICC/IF 1/500 - 1/2500.

Donkey Anti-Goat IgG H&L (HRP) preadsorbed images

  • Ab7125 was used at dilution 1/10000 with the primary antibody ab37917 in WB. See the review on ab37917.

References for Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125)

This product has been referenced in:
  • Du Roure C  et al. Hematopoietic Overexpression of FOG1 Does Not Affect B-Cells but Reduces the Number of Circulating Eosinophils. PLoS One 9:e92836 (2014). WB . Read more (PubMed: 24747299) »
  • Maekawa T  et al. Coiled-Coil Domain-Dependent Homodimerization of Intracellular Barley Immune Receptors Defines a Minimal Functional Module for Triggering Cell Death. Cell Host Microbe 9:187-99 (2011). WB . Read more (PubMed: 21402358) »

See all 3 Publications for this product

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Our distributor in Saudi-Arabia:

Abdulla Fouad Holding Co.
Abdulla Fouad Head Quarter Bldg.
Dammam-Khobar Highway
P.O. Box 257, Dammam
31411
Saudi Arabia
Tel. +9...

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Ab7125 has only been pre-adsorbed to the following: Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins. So, it most likely is binding non-specifically to proteins in your lysates. If you have any more questions, please contact us...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"