Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab97120)

Overview

  • Product name
    Donkey Anti-Goat IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP), pre-adsorbed
  • Target species
    Goat
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.
  • Tested applications
    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Minimal
    cross-reactivity

    Chicken, Human, Mouse, Pig, Rabbit, Rat more details
  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (Primary)
WB 1/5000 - 1/50000. Colorimetric 1/5000 - 1/30000; Chemiluminescent 1/10000 - 1/50000

References for Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab97120)

This product has been referenced in:
  • Ram RS  et al. Cancer Stem Cells in Moderately Differentiated Lip Squamous Cell Carcinoma Express Components of the Renin-Angiotensin System. Front Surg 4:30 (2017). WB ; Human . Read more (PubMed: 28634582) »
  • Itinteang T  et al. Cancer stem cells in moderately differentiated oral tongue squamous cell carcinoma express components of the renin-angiotensin system. J Clin Pathol 69:942-5 (2016). Read more (PubMed: 27371611) »

See all 6 Publications for this product

Product Wall



I am happy to confirm that we have a anti goat antibody that was pre-absorped against sheep IgG:

ab99713

http://www.abcam.com/index.html?datasheet=99713 (or use the following: http://www.abcam.com/index.html?datasheet=9971...

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Thank you for contacting Abcam.


Attached please find the CoC document you requested.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Thank you for contacting us. I have read the whole protocol you have kindly submitted. In order to address the problem quickly I have further questions to ask. - According to the attached image you have used non transfected N2a mouse cells, Lym...

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Thank you for contacting Abcam.   I have attached the MSDS for ab97120 and ab97105, both of which contain the preservative proclin.  A MSDS is not required for ab105466.   I hope this information is helpful.  Please do not hesitate to contact us if you...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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