Donkey Anti-Human IgG H&L preadsorbed (ab102421)

Overview

  • Product nameDonkey Anti-Human IgG H&L preadsorbed
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Human IgG - H&L, pre-adsorbed
  • Target speciesHuman
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, mouse, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.
  • Tested applicationsSuitable for: ICC, IHC-P, ELISA, WBmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Mouse, Rabbit, Rat, Sheep more details

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross adsorbed using bovine, chicken, goat, horse, mouse, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/100 - 1/30000. ELISA - Coating: 1/100 - 1/500; ELISA - Primary: 1/1000 - 1/30000
WB 1/1000 - 1/30000. WB - Colorimetric: 1/1000 - 1/10000; WB - Chemiluminescent: 1/1000 - 1/30000

References for Donkey Anti-Human IgG H&L preadsorbed (ab102421)

ab102421 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"