Donkey Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed (ab7062)

Overview

  • Product name
    Donkey Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed
    See all IgG secondary antibodies
  • Target species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopymore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details
  • Immunogen

    Full length native mouse IgG (purified).

  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 50% Glycerol, 10mg/ml BSA, 0.15M Sodium chloride, 0.05M Tris HCl, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 8.0
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads.
  • Conjugation notes
    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
  • Application notes
    Dot: Use at an assay dependant dilution.
    ELISA: 1/500 - 1/2000.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    IM: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    ab7062 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for your inquiry.
    There may be a problem in using the primary antibody ab24470 with a secondary antibody as it is already HRP conjugated itself. The steric hindrance created by this conjugate may hinder the efficient binding of the secon...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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