Donkey Anti-Mouse IgG H&L (Biotin) preadsorbed (ab7060)

Overview

  • Product name
    Donkey Anti-Mouse IgG H&L (Biotin) preadsorbed
    See all IgG secondary antibodies
  • Target species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopymore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human, Rabbit, Rat, Sheep more details
  • Immunogen

    Full length native mouse IgG (purified).

  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
  • Application notes
    Dot: Use at an assay dependant dilution.
    ELISA: 1/50000 - 1/200000.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    IM: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Donkey Anti-Mouse IgG H&L (Biotin) preadsorbed (ab7060)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Unfortunatley we do not have country of origin certificates for our products nor do we offer six months warranty. Our guarentee is that an antibody should work as stated on the data sheet or we will refund or replace it. I also cannot hazard a guess at...

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    This antibody is pre-adsorbed against Golden Syrian Hamster serum proteins. The cross reactivity to this species is very minimized. Cross-reactivity to Armenian hamster is unknown.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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