Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)


  • Product nameDonkey Anti-Mouse IgG H&L (DyLight® 550)
    See all IgG secondary antibodies
  • Target speciesMouse
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cyt, WBmore details
  • ConjugationDyLight® 550. Ex: 562nm, Em: 576nm


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notesDyLight® 550 replaces DyLight® 549.
  • Research areas


Our Abpromise guarantee covers the use of ab96876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500. DyLight® 550 replaces DyLight®549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight®550 provides the same orange-to-red fluorescence and photostability as the DyLight®549 dye. Please refer to the vial in order to check whether the product you received is DyLight®549 or DyLight®550 – this information however will have no impact on your experiments. For more information please refer to
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.
WB Use at an assay dependent concentration.

Donkey Anti-Mouse IgG H&L (DyLight® 550) images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.
  • Courtesy of an anonymous Abreview

References for Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)

This product has been referenced in:
  • de la Mare JA  et al. Extracellular Hsp90 and TGFß regulate adhesion, migration and anchorage independent growth in a paired colon cancer cell line model. BMC Cancer 17:202 (2017). Read more (PubMed: 28302086) »
  • Ferron PJ  et al. Comparative analysis of the cytotoxic effects of okadaic acid-group toxins on human intestinal cell lines. Mar Drugs 12:4616-34 (2014). ICC/IF . Read more (PubMed: 25196936) »

See all 2 Publications for this product

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