Donkey Anti-Mouse IgG H&L (DyLight® 650) preadsorbed (ab98797)

Overview

  • Product name
    Donkey Anti-Mouse IgG H&L (DyLight® 650) preadsorbed
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 650), pre-adsorbed
  • Target species
    Mouse
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep more details
  • Conjugation
    DyLight® 650. Ex: 654nm, Em: 673nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    DyLight® 650 replaces DyLight® 649.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500. DyLight®650 replaces DyLight®649. The DyLight®650 excitation is 652nm and the emission is 672nm. DyLight®650 hence provides the same far-red fluorescence and photostability as the DyLight®649 dye. Please refer to the vial in order to check whether the product you received is DyLight®649 or DyLight®650. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight650.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:
  • Löffler T  et al. Decreased Plasma Aß in Hyperlipidemic APPSL Transgenic Mice Is Associated with BBB Dysfunction. Front Neurosci 10:232 (2016). IHC-Fr . Read more (PubMed: 27313503) »
  • Cottle DL  et al. c-MYC-induced sebaceous gland differentiation is controlled by an androgen receptor/p53 axis. Cell Rep 3:427-41 (2013). Read more (PubMed: 23403291) »

See all 2 Publications for this product

Customer reviews and Q&As

Abreviews
Application
Immunohistochemistry (Frozen sections)
1:200 dilution
murine brain , frozen sections
1 hr at RT

From Abcam Scientific Support: Thank you for your valuable feedback. This abreview was submitted as part of our abtrial program as this antibody has not been previously tested and guaranteed to work in IHC-Fr. We welcome other abreviews to determine whether this antibody is suitable for this application.
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Mrs. gali umschweif

Verified customer

Submitted Dec 02 2013

Thank you for contacting us and your interest in our products.

Either ab98797 or ab96878 or should be compatible with the experiments you areintending toperform but I would suggest ab98797 maybe more appropriate. Ab98797 is different to ab968...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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