Donkey Anti-Mouse IgG H&L (FITC) (ab97029)

Overview

  • Product nameDonkey Anti-Mouse IgG H&L (FITC)
    See all IgG secondary antibodies
  • Target speciesMouse
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against nonimmunoglobulin serum proteins.
  • Tested applicationsIHC (PFA fixed), IHC-P, Flow Cyt, ICC/IFmore details
  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.
  • Conjugation notesF/P ratio is 3-7
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed) 1/100.
IHC-P 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.

References for Donkey Anti-Mouse IgG H&L (FITC) (ab97029)

ab97029 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA fixed)
Application: Immunofluorescence
Blocking step: BSA as blocking agent for 60 min • Concentration: 3% • Temperature: 25°C
Sample: confluent MDCK II cells (Canine kidney epithelial)
Specification: Dilution of Occludin antibody 1:50 and ab97029 antibody 1:100
Permeabilization: Yes - 0.3% Triton X 100
Fixative: 4%PFA
Username

Mr. Adel Hama

Verified customer

Submitted May 07 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"