Overview

  • Product name
    Donkey Anti-Mouse IgG H&L (FITC)
    See all IgG secondary antibodies
  • Host species
    Donkey
  • Target species
    Mouse
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against nonimmunoglobulin serum proteins.
  • Tested applications
    Suitable for: IHC (PFA fixed), IHC-P, Flow Cyt, ICC/IFmore details
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.
  • Conjugation notes
    F/P ratio is 3-7
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed) 1/100.
IHC-P 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.

References

This product has been referenced in:
  • Tolani B  et al. Preclinical characterization of therapeutic antibodies targeted at the carboxy-terminus of Sonic hedgehog. Oncotarget 9:14311-14323 (2018). Read more (PubMed: 29581846) »
  • Leprieur EG  et al. Membrane-bound full-length Sonic Hedgehog identifies cancer stem cells in human non-small cell lung cancer. Oncotarget 8:103744-103757 (2017). Read more (PubMed: 29262597) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (PFA fixed)
Application: Immunofluorescence
Blocking step: BSA as blocking agent for 60 min • Concentration: 3% • Temperature: 25°C
Sample: confluent MDCK II cells (Canine kidney epithelial)
Specification: Dilution of Occludin antibody 1:50 and ab97029 antibody 1:100
Permeabilization: Yes - 0.3% Triton X 100
Fixative: 4%PFA
Username

Mr. Adel Hama

Verified customer

Submitted May 07 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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