Overview

  • Product nameDonkey Anti-Mouse IgG H&L (HRP)
    See all IgG secondary antibodies
  • Target speciesMouse
  • Tested applicationsSuitable for: Dot blot, ELISA, IHC-P, IHC-Fr, WB, ICC/IFmore details
  • Immunogen

    mouse IgG whole molecule

  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.2
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesHorseradish Peroxidase (HRP)
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
ELISA 1/70000.
IHC-P 1/500 - 1/2000.
IHC-Fr 1/500 - 1/2000.
WB 1/2000 - 1/10000.
ICC/IF 1/500 - 1/2500.

Donkey Anti-Mouse IgG H&L (HRP) images

  • IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6820, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References for Donkey Anti-Mouse IgG H&L (HRP) (ab6820)

This product has been referenced in:
  • Benz N  et al. Improvement of chloride transport defect by gonadotropin-releasing hormone (GnRH) in cystic fibrosis epithelial cells. PLoS One 9:e88964 (2014). WB . Read more (PubMed: 24586461) »
  • Abdulsid A  et al. Heat shock protein 27 is spatially distributed in the human placenta and decreased during labor. PLoS One 8:e71127 (2013). WB . Read more (PubMed: 23990930) »

See all 3 Publications for this product

Product Wall

Thank you very much for your reply.

I am sorry that the staining using the Donkey polyclonal Secondary Antibody to Mouse IgG (ab6820) has not performed as expected in your experiment. My aim to gather further information relating to the proto...

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Thank you for your reply.

I can understand your concern and my aim is to make sure that we resolve this situation as quickly as possible so that you are able to get on with your research. However, in order to achieve this we need to establish...

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Thank you for your very detailed reply and sorry for my late reply.

I have been looking into the problems you have encountered and am still investigating the case. I thinkwith the protocol you have been using that there should be staining. Ho...

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Thank you for taking time to completethequestionnaire. The details provided will enableme to investigate this case and will provide us with vital information for monitoring product quality.

Having reviewed the information you have provided t...

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Thank you for calling us today and reporting the problem you have beenexperiencing with the Anti-COX1 / Cyclooxygenase 1 antibody [CYO-1] (ab22696)used incombination with the Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6820). W...

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Thank you for contacting us.

we tested catalog ab126709in IP, IHC, ICC, and Flow cytometry and it is tested negative. For this reason, we only recommending the product for western blot.

I hope this information is helpful to you. Pleas...

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Thank you for contacting us.

The ab22696 could be used at 1/10 - 1/100 dilution.
ab54615 at 5-10ug/ml
ab103724 at 5-10ug/ml
ab126709 at 1/500-1/1000 dilution.

ab6820 and ab6802 at 1/500-1/2000 dilution.

I hope thi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"