Donkey Anti-Mouse IgG H&L (HRP) (ab97030)
Key features and details
- Donkey Anti-Mouse IgG H&L (HRP)
- Conjugation: HRP
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Overview
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Product name
Donkey Anti-Mouse IgG H&L (HRP)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Mouse -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against nonimmunoglobulin serum proteins. -
Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
Conjugation notes
Molar enzyme/ antibody protein ratio is 4:1 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent dilution.
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IHC-P |
1/200 - 1/5000.
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ELISA |
1/1000 - 1/100000. (Primary)
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|
WB |
1/5000 - 1/30000. (Colorimetric) 1/1000 - 1/50000 (Chemiluminescent)
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Notes |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/5000. |
ELISA
1/1000 - 1/100000. (Primary) |
WB
1/5000 - 1/30000. (Colorimetric) 1/1000 - 1/50000 (Chemiluminescent) |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab97030 has been referenced in 14 publications.
- Bhattarai U et al. IL-12α deficiency attenuates pressure overload-induced cardiac inflammation, hypertrophy, dysfunction, and heart failure progression. Front Immunol 14:1105664 (2023). PubMed: 36860846
- Niu F et al. The m6A reader YTHDF2 is a negative regulator for dendrite development and maintenance of retinal ganglion cells. Elife 11:N/A (2022). PubMed: 35179492
- Otte K et al. Eltanexor Effectively Reduces Viability of Glioblastoma and Glioblastoma Stem-Like Cells at Nano-Molar Concentrations and Sensitizes to Radiotherapy and Temozolomide. Biomedicines 10:N/A (2022). PubMed: 36140245
- Zhao K et al. Inhibition of Carbonic Anhydrase 2 Overcomes Temozolomide Resistance in Glioblastoma Cells. Int J Mol Sci 23:N/A (2021). PubMed: 35008590
- Dai XW et al. lncRNA-MIAT facilitates the differentiation of adipose-derived mesenchymal stem cells into lymphatic endothelial cells via the miR-495/Prox1 axis. Mol Med Rep 23:N/A (2021). PubMed: 33760182