Donkey Anti-Mouse IgG H&L (HRP) preadsorbed (ab7061)

Overview

  • Product name
    Donkey Anti-Mouse IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Target species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopymore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details
  • Immunogen

    Full length native mouse IgG (purified).

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Horseradish Peroxidase (HRP)
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/20000.
ICC/IF 1/1000 - 1/5000.
Dot blot Use at an assay dependent dilution.
ELISA 1/140000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.

References

This product has been referenced in:
  • Kolyada A  et al. Inhibition of thrombotic properties of persistent autoimmune anti-ß2GPI antibodies in the mouse model of antiphospholipid syndrome. Blood 123:1090-7 (2014). Read more (PubMed: 24277078) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting us.

The following products would be suitable as per your requirements. The catalogue numbers are

ab5870, ab5887, ab7061, ab7068, ab98717, ab98790 etc

I hope this information is helpful to you. Pl...

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Thank you for your enquiry. I am sorry to hear that you have been obtaining high background with this antibody. Following contact with the source of this antibody I have determined that the antibody is affinity purified, then cross adsorbed using so...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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