Donkey Anti-Mouse IgG H&L preadsorbed (ab7056)
Key features and details
- Donkey Anti-Mouse IgG H&L preadsorbed
- Host species: Donkey
- Suitable for: WB, IP, ICC/IF, Immunodiffusion, Conjugation, IHC-P, IHC-Fr, ELISA
Related conjugates and formulations
Overview
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Product name
Donkey Anti-Mouse IgG H&L preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Mouse -
Tested applications
Suitable for: WB, IP, ICC/IF, Immunodiffusion, Conjugation, IHC-P, IHC-Fr, ELISAmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details -
Immunogen
Full length native Mouse IgG (purified).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000.
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|
IP |
Use at an assay dependent concentration.
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|
ICC/IF |
1/1000 - 1/5000.
|
|
Immunodiffusion |
Use at an assay dependent concentration.
|
|
Conjugation |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent concentration.
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
|
ELISA |
1/30000 - 1/120000.
|
Notes |
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WB
1/2000 - 1/10000. |
IP
Use at an assay dependent concentration. |
ICC/IF
1/1000 - 1/5000. |
Immunodiffusion
Use at an assay dependent concentration. |
Conjugation
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ELISA
1/30000 - 1/120000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab7056 has been referenced in 2 publications.
- Wu X et al. In situ characterization of the mTORC1 during adipogenesis of human adult stem cells on chip. Proc Natl Acad Sci U S A 113:E4143-50 (2016). WB . PubMed: 27382182
- Lindén S et al. Terbium-based time-gated Förster resonance energy transfer imaging for evaluating protein-protein interactions on cell membranes. Dalton Trans 44:4994-5003 (2015). PubMed: 25612290