Donkey Anti-Mouse IgG H&L (Texas Red ®) preadsorbed (ab7059)

Overview

  • Product nameDonkey Anti-Mouse IgG H&L (Texas Red ®) preadsorbed
    See all IgG secondary antibodies
  • Target speciesMouse
  • Tested applicationsSuitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA, ICC/IFmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details
  • Immunogen

    Full length native mouse IgG (purified).

  • ConjugationTexas Red ®. Ex: 596nm, Em: 620nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesTexas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG
  • ClonalityPolyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.
ICC/IF 1/1000 - 1/5000. PubMed: 18263878

References for Donkey Anti-Mouse IgG H&L (Texas Red ®) preadsorbed (ab7059)

This product has been referenced in:
  • Planutis K  et al. A novel signaling pathway regulates colon cancer angiogenesis through Norrin. Sci Rep 4:5630 (2014). IHC-P ; Human . Read more (PubMed: 25005225) »
  • Hejna J  et al. Tip60 Is Required for DNA Interstrand Cross-link Repair in the Fanconi Anemia Pathway. J Biol Chem 283:9844-51 (2008). ICC/IF . Read more (PubMed: 18263878) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.
I am sorry to hear that you received so far no staining with the antibodies.

As we discussed by phone, I'd suggest trying the following:

1) try another antigen retrieval step (e.g. Tris/EDTA, pH 8) ...

Read More

This antibody will detect IgG1 kappa.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"