Donkey Anti-Mouse IgG H&L (TRITC) preadsorbed (ab7058)

Overview

  • Product nameDonkey Anti-Mouse IgG H&L (TRITC) preadsorbedSee all IgG secondary antibodies ...
  • Description
    Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (TRITC ), pre-adsorbed
  • Target speciesMouse
  • Tested applicationsICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA more details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details
  • Immunogen

    Full length native Mouse IgG (purified).

  • ConjugationTRITC. Ex: 547nm, Em: 572nm

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesTetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.8 moles TRITC per mole of Rabbit IgG
  • Clonality Polyclonal
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab7058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF
Immunomicroscopy
Flow Cyt
IHC-P
IHC-Fr
ELISA
  • Application notesFlow Cyt: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    IM: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Donkey Anti-Mouse IgG H&L (TRITC) preadsorbed (ab7058)

    ab7058 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"