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Product name
Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (AP), pre-adsorbed
See all IgG products (1703) ...
Target species
Rabbit
Tested applications
ICC/IF, IHC-P, WB, ELISA, Dot Blot, IHC-Frmore details
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheepmore details
Immunogen
Rabbit IgG whole molecule
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Conjugation notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Clonality
Polyclonal
Research areas
Secondary antibodies >> anti-Rabbit >> IgG >> Enzyme >> Alkaline Phos
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
ICC/IF: 1/400 - 1/2000.ICC/IF: 1/400 - 1/2000.
IHC-P: Use at an assay dependent concentration.
WB
WB: 1/1000 - 1/5000.WB: 1/1000 - 1/5000.
ELISA: 1/4000 - 1/16000.
Dot: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
References for Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (AP), pre-adsorbed (ab7084)
This product has been referenced in:
- Peng EW et al. Myocardial expression of heat shock protein 70i protects early postoperative right ventricular function in cyanotic tetralogy of Fallot. J Thorac Cardiovasc Surg 141:1184-91 (2011).Read more (PubMed: 21397265) »
See 1 publication for this product
Publishing research using ab7084? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"