Overview

  • Product name
    Donkey Anti-Rabbit IgG H&L (10nm Gold)
    See all IgG secondary antibodies
  • Target species
    Rabbit
  • Tested applications
    Suitable for: Electron Microscopymore details
  • Immunogen

    Rabbit IgG whole molecule.

  • Conjugation
    Gold 10nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This product has been immuno-affinity purified and immuno cross-absorbed to reduce non-specific reactions. The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes

    This product is an immunogold reagent built around colloidal gold particles of 10nm. The paritcle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 10nm particle size conjugate is less than 10 %.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab39597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
  • Application notes
    Electron Microscopy: Use at an assay dependent dilution.
    Light Microscopy: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Donkey Anti-Rabbit IgG H&L (10nm Gold) (ab39597)

    This product has been referenced in:
    • Goupil LS  et al. Cysteine and Aspartyl Proteases Contribute to Protein Digestion in the Gut of Freshwater Planaria. PLoS Negl Trop Dis 10:e0004893 (2016). Read more (PubMed: 27501047) »

    See 1 Publication for this product

    Product Wall

    Le secondaire ab39597 couplé aux billes d'or de 10nm a les caratéristiques suivantes :

    3.3 x 1011 particules d'or / ml
    115-180 anticorps / bille d'or
    La concentration d'anticorps est d'environ 10 µg/ml


    1. ces réactifs n'ont pas de concentration déterminée mais ils sont fournis à une DO de 1.0 à 520nm,
    2. la concentration en anticorps est comprise entre 5 et 10 µg/ml,
    3. Une particule d'or de 10nm ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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