Donkey Anti-Rabbit IgG H&L (25nm Gold) (ab41514)

Overview

  • Product name
    Donkey Anti-Rabbit IgG H&L (25nm Gold)
    See all IgG secondary antibodies
  • Target species
    Rabbit
  • Tested applications
    Suitable for: Electron Microscopymore details
  • Immunogen

    Rabbit IgG whole molecule.

  • Conjugation
    Gold 25nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This product has been immuno-affinity purified and immuno cross-absorbed to reduce non-specific reactions. The activity of each lot is determined using a dot-spot test system [Moeremans et al., J. Immunol., Methods 74, (1984), 353.]
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes

    This product is an immunogold reagent built around colloidal gold particles of 25nm. The particle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coeffiecient of variance for the 25nm particle size conjugate is less than 12%.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab41514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
  • Application notes
    Electron Microscopy: Use at an assay dependent dilution. The method of production of this product allows the small particle size of 25nm to be used instead of 5nm with the same sensitivity but increased visibility in the standard transmission electron microscope (See also ven Bergen en Henegouwen and Leunissen, Histochemistry, 85,(1986), 81.)

    Light Microscopy: Use at an assay dependent dilution.



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    ab41514 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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