AbExcel

Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97061)

Overview

  • Product nameDonkey Anti-Rabbit IgG H&L (Alkaline Phosphatase)See all IgG secondary antibodies ...
  • Target speciesRabbit
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • Tested applicationsICC, ELISA, WB, IHC-Pmore details
  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
  • Clonality Polyclonal
  • IsotypeIgG
  • General notesPart of the AbExcel range.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
ELISA 1/5000 - 1/50000.
WB 1/5000 - 1/50000.
IHC-P 1/100 - 1/1000.

Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) images

  • All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (Alkaline Phosphatase) (ab97061) at 1/5000 dilution
    Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (Alkaline Phosphatase) (ab97061) at 1/20000 dilution
    Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (Alkaline Phosphatase) (ab97061) at 1/50000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 3 minutes

References for Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97061)

ab97061 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

The link to our indirect ELISA protocol is:
http://www.abcam.com/index.html?pageconfig=resource&rid=11389

I have also attached the protocol as pdf-file.

I hope this information is h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"