Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed (ab97082)

Overview

  • Product nameDonkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (AP), pre-adsorbed
  • Target speciesRabbit
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
  • Tested applicationsSuitable for: ICC, WB, IHC-P, ELISAmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Human, Mouse, Pig, Rat more details
  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
WB 1/2500 - 1/25000. (Chemiluminescent)
IHC-P 1/100 - 1/1000.
ELISA 1/2500 - 1/25000. (Primary ELISA)

References for Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed (ab97082)

ab97082 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"