Overview

  • Product name
    Donkey Anti-Rabbit IgG H&L (Biotin)
    See all IgG secondary antibodies
  • Target species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
  • Immunogen

    Rabbit IgG whole molecule

  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Donkey IgG molecule.
  • Conjugation notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/3000 - 1/15000.
ELISA 1/150000.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/500 - 1/3000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Images

  • Ab6801 was used at dilution 1/200 with the primary antibody ab64869 in IHC-Fr. See the review on ab64869.
  • Ab6801 was used at dilution 1/200 with the primary antibody ab39350 in IHC - Free Floating. See the review on ab39350.

References

This product has been referenced in:
  • Johnson MB  et al. Topical Fibronectin Improves Wound Healing of Irradiated Skin. Sci Rep 7:3876 (2017). IHC-P ; Mouse . Read more (PubMed: 28634413) »
  • Ghorbani M  et al. Apparent histological changes of adipocytes after treatment with CL 316,243, a ß-3-adrenergic receptor agonist. Drug Des Devel Ther 9:669-76 (2015). IHC-P . Read more (PubMed: 25709398) »

See all 8 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab6801.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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