Overview

  • Product nameDonkey Anti-Rabbit IgG H&L (Biotin)
    See all IgG secondary antibodies
  • Target speciesRabbit
  • Tested applicationsSuitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
  • Immunogen

    Rabbit IgG whole molecule

  • ConjugationBiotin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Donkey IgG molecule.
  • Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/3000 - 1/15000.
ELISA 1/150000.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/500 - 1/3000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Donkey Anti-Rabbit IgG H&L (Biotin) images

  • Ab6801 was used at dilution 1/200 with the primary antibody ab64869 in IHC-Fr. See the review on ab64869.
  • Ab6801 was used at dilution 1/200 with the primary antibody ab39350 in IHC - Free Floating. See the review on ab39350.

References for Donkey Anti-Rabbit IgG H&L (Biotin) (ab6801)

This product has been referenced in:
  • Ghorbani M  et al. Apparent histological changes of adipocytes after treatment with CL 316,243, a ß-3-adrenergic receptor agonist. Drug Des Devel Ther 9:669-76 (2015). IHC-P . Read more (PubMed: 25709398) »
  • Jung CS  et al. Endogenous nitric-oxide synthase inhibitor ADMA after acute brain injury. Int J Mol Sci 15:4088-103 (2014). IHC-Fr . Read more (PubMed: 24663083) »

See all 4 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab6801.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"