Overview

  • Product name
    Donkey Anti-Rabbit IgG H&L (Biotin)
    See all IgG secondary antibodies
  • Target species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
  • Immunogen

    Rabbit IgG whole molecule

  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Donkey IgG molecule.
  • Conjugation notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/3000 - 1/15000.
ELISA 1/150000.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/500 - 1/3000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Donkey Anti-Rabbit IgG H&L (Biotin) images

  • Ab6801 was used at dilution 1/200 with the primary antibody ab64869 in IHC-Fr. See the review on ab64869.
  • Ab6801 was used at dilution 1/200 with the primary antibody ab39350 in IHC - Free Floating. See the review on ab39350.

References for Donkey Anti-Rabbit IgG H&L (Biotin) (ab6801)

This product has been referenced in:
  • Johnson MB  et al. Topical Fibronectin Improves Wound Healing of Irradiated Skin. Sci Rep 7:3876 (2017). IHC-P ; Mouse . Read more (PubMed: 28634413) »
  • Ghorbani M  et al. Apparent histological changes of adipocytes after treatment with CL 316,243, a ß-3-adrenergic receptor agonist. Drug Des Devel Ther 9:669-76 (2015). IHC-P . Read more (PubMed: 25709398) »

See all 8 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab6801.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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