Donkey Anti-Rabbit IgG H&L (Biotin) preadsorbed (ab97083)
Key features and details
- Donkey Anti-Rabbit IgG H&L (Biotin) preadsorbed
- Conjugation: Biotin
- Host species: Donkey
- Isotype: IgG
- Suitable for: WB, IHC-P, ICC/IF, ELISA
Overview
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Product name
Donkey Anti-Rabbit IgG H&L (Biotin) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
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Tested applications
Suitable for: WB, IHC-P, ICC/IF, ELISAmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Human, Mouse, Pig, Rat more details -
Immunogen
Other Immunogen Type corresponding to Goat IgG.
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/200000. (Chemiluminescent)
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IHC-P |
1/250 - 1/2500.
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ICC/IF |
1/100 - 1/500.
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ELISA |
1/10000 - 1/200000. (Primary ELISA)
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Notes |
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WB
1/10000 - 1/200000. (Chemiluminescent) |
IHC-P
1/250 - 1/2500. |
ICC/IF
1/100 - 1/500. |
ELISA
1/10000 - 1/200000. (Primary ELISA) |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (4)
ab97083 has been referenced in 4 publications.
- Ma P et al. RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission. Sci Adv 8:eabq4736 (2022). PubMed: 36179027
- Lagedal R et al. Impaired Antibody Response Is Associated with Histone-Release, Organ Dysfunction and Mortality in Critically Ill COVID-19 Patients. J Clin Med 11:N/A (2022). PubMed: 35743491
- Huckriede J et al. Evolution of NETosis markers and DAMPs have prognostic value in critically ill COVID-19 patients. Sci Rep 11:15701 (2021). PubMed: 34344929
- Huckriede J et al. Histone H3 Cleavage in Severe COVID-19 ICU Patients. Front Cell Infect Microbiol 11:694186 (2021). PubMed: 34568088