Donkey anti-Rabbit IgG H&L (DyLight® 488) secondary antibody (ab98488)

Overview

  • Product name
    Donkey anti-Rabbit IgG H&L (DyLight® 488) secondary antibody
    See all IgG secondary antibodies
  • Description
    Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed
  • Target species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Human, Mouse, Rat, Sheep more details
  • Conjugation
    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 488.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.

Images

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Rabbit anti-laminin a1 (basement membrane marker), 1:400

    Secondary antibody: Donkey F(ab')2 polyclonal to Rabbit IgG - H&L DyLight® 488 (ab98488), 1:200

    F-actin was stained with rhodamine-phallodoin 1:100
    Nuclei were counterstained with DAPI
  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:
  • Xia F  et al. IRGM1 regulates oxidized LDL uptake by macrophage via actin-dependent receptor internalization during atherosclerosis. Sci Rep 3:1867 (2013). Read more (PubMed: 23689639) »
  • Wang W  et al. Overexpression of Uromodulin-like1 accelerates follicle depletion and subsequent ovarian degeneration. Cell Death Dis 3:e433 (2012). Read more (PubMed: 23190605) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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