Overview

  • Product name
    Donkey Anti-Rabbit IgG H&L (DyLight® 550)
    See all IgG secondary antibodies
  • Target species
    Rabbit
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details
  • Conjugation
    DyLight® 550. Ex: 562nm, Em: 576nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    DyLight® 550 replaces DyLight® 549.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500. DyLight® 550 replaces DyLight®549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight®550 provides the same orange-to-red fluorescence and photostability as the DyLight®549 dye. Please refer to the vial in order to check whether the product you received is DyLight®549 or DyLight®550. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight550.

Donkey Anti-Rabbit IgG H&L (DyLight® 550) images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Donkey Anti-Rabbit IgG H&L (DyLight® 550) (ab96892)

This product has been referenced in:
  • de la Mare JA  et al. Extracellular Hsp90 and TGFß regulate adhesion, migration and anchorage independent growth in a paired colon cancer cell line model. BMC Cancer 17:202 (2017). Read more (PubMed: 28302086) »
  • Cen M  et al. TIEG1 deficiency confers enhanced myocardial protection in the infarcted heart by mediating the Pten/Akt signalling pathway. Int J Mol Med 39:569-578 (2017). Read more (PubMed: 28204828) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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