Donkey anti-Rabbit IgG H&L (DyLight® 550) secondary antibody (ab98489)

Overview

  • Product name
    Donkey anti-Rabbit IgG H&L (DyLight® 550) secondary antibody
    See all IgG secondary antibodies
  • Description
    Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 550), pre-adsorbed
  • Target species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Human, Mouse, Rat, Sheep more details
  • Conjugation
    DyLight® 550. Ex: 562nm, Em: 576nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 550.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    DyLight® 550 replaces DyLight® 549.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500. DyLight®550 replaces DyLight®549. The DyLight®550 excitation is 562nm and the emission is 576nm. DyLight®550 provides the same orange-to-red fluorescence and photostability as the DyLight®549 dye. Please refer to the vial in order to check whether the product you received is DyLight®549 or DyLight®550. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight550.

Donkey anti-Rabbit IgG H&L (DyLight® 550) secondary antibody images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Donkey anti-Rabbit IgG H&L (DyLight® 550) secondary antibody (ab98489)

This product has been referenced in:
  • Cervantes S  et al. The multifunctional autophagy pathway in the human malaria parasite, Plasmodium falciparum. Autophagy 10:80-92 (2014). Read more (PubMed: 24275162) »
  • He S  et al. Immune-related GTPase M (IRGM1) regulates neuronal autophagy in a mouse model of stroke. Autophagy 8:1621-7 (2012). Read more (PubMed: 22874556) »

See all 2 Publications for this product

Product Wall

Thank you for contacting Abcam regarding ab98489.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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