Donkey Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed (ab96922)

Overview

  • Product nameDonkey Anti-Rabbit IgG H&L (DyLight® 650) preadsorbedSee all IgG secondary antibodies ...
  • Description
    Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 650), pre-adsorbed
  • Target speciesRabbit
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
  • Tested applicationsICC/IF, IHC-P, Flow Cytmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details
  • ConjugationDyLight® 650. Ex: 654nm, Em: 673nm

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
  • Clonality Polyclonal
  • IsotypeIgG
  • General notesDyLight® 650 replaces DyLight® 649.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab96922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500. DyLight®650 replaces DyLight®649. The DyLight®650 excitation is 652nm and the emission is 672nm. DyLight®650 hence provides the same far-red fluorescence and photostability as the DyLight®649 dye. Please refer to the vial in order to check whether the product you received is DyLight®649 or DyLight®650. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight650.
Flow Cyt 1/50 - 1/200.

Donkey Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Donkey Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed (ab96922)

This product has been referenced in:
  • Gunness P  et al. 3D organotypic cultures of human HepaRG cells: a tool for in vitro toxicity studies. Toxicol Sci 133:67-78 (2013). Read more (PubMed: 23377618) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"