Donkey Anti-Rabbit IgG H&L (FITC) preadsorbed (ab97084)

Overview

  • Product name
    Donkey Anti-Rabbit IgG H&L (FITC) preadsorbed
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC), pre-adsorbed
  • Target species
    Rabbit
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
  • Tested applications
    Suitable for: ICC/IF, IHC-P, Flow Cytmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Human, Mouse, Pig, Rat more details
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

References for Donkey Anti-Rabbit IgG H&L (FITC) preadsorbed (ab97084)

This product has been referenced in:
  • Lajko M  et al. Photoreceptor oxidative stress in hyperoxia-induced proliferative retinopathy accelerates rd8 degeneration. PLoS One 12:e0180384 (2017). IHC-P ; Mouse . Read more (PubMed: 28671996) »

See 1 Publication for this product

Product Wall

We used the following protocol with Anti-Glucose Transporter GLUT4 antibody (ab62375):

NSO cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat...

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Thank you for contacting us in regards to pre-incubating a primary and secondary together prior to performing FACS analysis.

I have spoken to my colleague who is quite experienced in using flow cytometry and she has informed me that whilst pr...

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Thank you for contacting us.
I am sorry to hear that you received so far no staining with the antibodies.

As we discussed by phone, I'd suggest trying the following:

1) try another antigen retrieval step (e.g. Tris/EDTA, pH 8) ...

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Thank you very much for contacting us.

Please find attached the QC ICC/IF image for this antibody. The cell line is A172 cells. The antibodies used is at 5ug//ml and detected using Alexa Fluor 488 conjugated secondary antibody. The nuclei are ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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