Overview

  • Product name
    Donkey Anti-Rabbit IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species
    Donkey
  • Target species
    Rabbit
  • Specificity
    Reacts with the heavy and light chains of the immunoglobulin molecule.
  • Tested applications
    Suitable for: WBmore details
  • Immunogen

    Synthetic peptide corresponding to IgG.

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, 10mg/ml BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    Part of the AbExcel range.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/20000. Optimized for ECL applications.

Images

  • Western blot - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)
    Western blot - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)
    All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/2000 dilution
    Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/10000 dilution
    Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time : 150 seconds
  • ELISA - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)
    ELISA - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)
    Ab16284 was used at dilution 1/5000 with the primary antibody ab52903 in ELISA. See the review on ab52903.
  • Western blot - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)
    Western blot - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)
    Ab16284 was used at dilution 1/2000 with the primary antibody ab28379 in WB. See the review on ab28379.

References

This product has been referenced in:
  • Cruz IN  et al. Proteomics Analysis of Ovarian Cancer Cell Lines and Tissues Reveals Drug Resistance-associated Proteins. Cancer Genomics Proteomics 14:35-51 (2017). Read more (PubMed: 28031236) »
  • Yang L  et al. AMP-activated protein kinase mediates the effects of lipoprotein-associated phospholipase A2 on endothelial dysfunction in atherosclerosis. Exp Ther Med 13:1622-1629 (2017). Read more (PubMed: 28413519) »

See all 25 Publications for this product

Publishing research using ab16284? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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3 reviews or Q&A

10
Votes: Highest First

WB with primary Ab to GAPDH (rabbit poly)
other secondary Abs work with this priamry
Ab: 1/10,000 - 1/2000 2h RT
block: 5% milk

We have issued a free of charge replacement.

I would like to inform you that I plan to use Peroxide/DAB the substrate and chromogen for horseradish peroxidase as detection system I appreciate you for your kind help                                                       with my best regards

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Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the c...

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I purchased ab9071 and ab9072 as they are noted on the Abcam website to be useful as a matched pair for sELISA. However the anti-mouse HRP secondary is binding to the capture antibody as well as the mouse detection antibody, producing a very high signa...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacements for one vial of ab63913 and one vial of ab16284 with the order number 1147505.
...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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