Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)

Overview

• Product nameDonkey Anti-Rabbit IgG H&L (HRP)
  See all IgG secondary antibodies
• Target speciesRabbit
• SpecificityReacts with the heavy and light chains of the immunoglobulin molecule.
• Tested applicationsSuitable for: WBmore details
• Immunogen

  Synthetic peptide corresponding to IgG.

• ConjugationHRP

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Store at +4°C.
• Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
  Constituents: 50% Glycerol, 10mg/ml BSA
• Concentration information loading...
• PurityProtein G purified
• ClonalityPolyclonal
• IsotypeIgG
• General notesPart of the AbExcel range.
• Research areas
  • Immunology
  • Immunoglobulins
  • Heavy Chain
  • IgG

  • Secondary antibodies
  • anti-Rabbit
  • IgG
  • Enzyme
  • HRP

Donkey Anti-Rabbit IgG H&L (HRP) images

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  Western blot - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)

  All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/2000 dilution Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/10000 dilution Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/20000 dilution Developed using the ECL technique. Performed under reducing conditions. Exposure time : 150 seconds

• 

  ELISA - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)
  Ab16284 was used at dilution 1/5000 with the primary antibody ab52903 in ELISA. See the review on ab52903.
• 

  Western blot - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)
  Ab16284 was used at dilution 1/2000 with the primary antibody ab28379 in WB. See the review on ab28379.