Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)
Overview
- Product nameDonkey Anti-Rabbit IgG H&L (HRP)See all IgG secondary antibodies ...
- DescriptionDonkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
- Target speciesRabbit
- SpecificityReacts with the heavy and light chains of the immunoglobulin molecule.
- Tested applicationsWB more details
- Immunogen
Synthetic peptide corresponding to IgG.
- ConjugationHRP
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 50% Glycerol, 10mg/ml BSA -
Concentration information loading... - PurityProtein G purified
- Clonality Polyclonal
- IsotypeIgG
- General notesPart of the AbExcel range.
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab16284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/2000 - 1/20000. Optimized for ECL applications. |
Donkey Anti-Rabbit IgG H&L (HRP) images
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Western blot - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.
Secondary
Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/2000 dilution
Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/10000 dilution
Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time : 150 seconds -
ELISA - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) -
Western blot - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)
References for Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)
This product has been referenced in:
- Malykhina AP et al. Bladder outlet obstruction triggers neural plasticity in sensory pathways and contributes to impaired sensitivity in erectile dysfunction. Am J Physiol Regul Integr Comp Physiol 304:R837-45 (2013). Read more (PubMed: 23535456) »
- Zschemisch NH et al. Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat. BMC Immunol 13:60 (2012). WB . Read more (PubMed: 23136839) »
