Synthetic peptide corresponding to IgG.
Our Abpromise guarantee covers the use of ab16284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/2000 - 1/20000. Optimized for ECL applications. |
All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.
Secondary Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/2000 dilution Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/10000 dilution Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/20000 dilution Developed using the ECL technique. Performed under reducing conditions. Exposure time : 150 seconds |
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"