Directly conjugated antibodies for immunofluorescence

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Overview

  • Product nameDonkey Anti-Rabbit IgG H&L (HRP)
    See all IgG secondary antibodies
  • Target speciesRabbit
  • SpecificityReacts with the heavy and light chains of the immunoglobulin molecule.
  • Tested applicationsWBmore details
  • Immunogen

    Synthetic peptide corresponding to IgG.

  • ConjugationHRP

Properties

Applications

Our Abpromise guarantee covers the use of ab16284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/20000. Optimized for ECL applications.

Donkey Anti-Rabbit IgG H&L (HRP) images

  • Western blot - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)
    Western blot - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)
    All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/2000 dilution
    Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/10000 dilution
    Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time : 150 seconds
  • ELISA - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)
    ELISA - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)
    Ab16284 was used at dilution 1/5000 with the primary antibody ab52903 in ELISA. See the review on ab52903.
  • Western blot - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)
    Western blot - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)
    Ab16284 was used at dilution 1/2000 with the primary antibody ab28379 in WB. See the review on ab28379.

References for Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)

This product has been referenced in:
  • Malykhina AP  et al. Bladder outlet obstruction triggers neural plasticity in sensory pathways and contributes to impaired sensitivity in erectile dysfunction. Am J Physiol Regul Integr Comp Physiol 304:R837-45 (2013). Read more (PubMed: 23535456) »
  • Alikhani M  et al. Expression analysis of RNA-binding motif gene on Y chromosome (RBMY) protein isoforms in testis tissue and a testicular germ cell cancer-derived cell line (NT2). Iran Biomed J 17:54-61 (2013). Read more (PubMed: 23567846) »

See all 15 Publications for this product

Publishing research using ab16284 ? Please let us know so that we can cite the reference in this datasheet

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3 Abreviews or Q&A

10
Votes: Highest First

WB with primary Ab to GAPDH (rabbit poly)
other secondary Abs work with this priamry
Ab: 1/10,000 - 1/2000 2h RT
block: 5% milk

We have issued a free of charge replacement.

I would like to inform you that I plan to use Peroxide/DAB the substrate and chromogen for horseradish peroxidase as detection system I appreciate you for your kind help                                                       with my best regards

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Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the c...

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I purchased ab9071 and ab9072 as they are noted on the Abcam website to be useful as a matched pair for sELISA. However the anti-mouse HRP secondary is binding to the capture antibody as well as the mouse detection antibody, producing a very high signa...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacements for one vial of ab63913 and one vial of ab16284 with the order number 1147505.
...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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