For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome.
Synthetic peptide corresponding to IgG.
Our Abpromise guarantee covers the use of ab16284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/20000. Optimized for ECL applications.|
All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/2000 dilution
Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/10000 dilution
Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time : 150 seconds
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"