Customer reviews and FAQs

Overview

  • Product name
    Donkey Anti-Rabbit IgG H&L (HRP)
    See all IgG secondary antibodies
  • Target species
    Rabbit
  • Specificity
    Reacts with the heavy and light chains of the immunoglobulin molecule.
  • Tested applications
    Suitable for: WBmore details
  • Immunogen

    Synthetic peptide corresponding to IgG.

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, 10mg/ml BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    Part of the AbExcel range.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/20000. Optimized for ECL applications.

Donkey Anti-Rabbit IgG H&L (HRP) images

  • Western blot - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)
    Western blot - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)
    All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/2000 dilution
    Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/10000 dilution
    Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time : 150 seconds
  • ELISA - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)
    ELISA - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)
    Ab16284 was used at dilution 1/5000 with the primary antibody ab52903 in ELISA. See the review on ab52903.
  • Western blot - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)
    Western blot - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)
    Ab16284 was used at dilution 1/2000 with the primary antibody ab28379 in WB. See the review on ab28379.

References for Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)

This product has been referenced in:
  • Mostyn A  et al. UCP1 is present in porcine adipose tissue and is responsive to postnatal leptin. J Endocrinol 223:M31-8 (2014). Read more (PubMed: 25122002) »
  • Fazeli AS  et al. Proteome analysis of post-transplantation recovery mechanisms of an EAE model of multiple sclerosis treated with embryonic stem cell-derived neural precursors. J Proteomics 94:437-50 (2013). Read more (PubMed: 23791935) »

See all 16 Publications for this product

Publishing research using ab16284? Please let us know so that we can cite the reference in this datasheet.

Product Wall

Submit an abreview for ab16284 Submit a question for ab16284

3 Abreviews or Q&A

10
Votes: Highest First

WB with primary Ab to GAPDH (rabbit poly)
other secondary Abs work with this priamry
Ab: 1/10,000 - 1/2000 2h RT
block: 5% milk

We have issued a free of charge replacement.

I would like to inform you that I plan to use Peroxide/DAB the substrate and chromogen for horseradish peroxidase as detection system I appreciate you for your kind help                                                       with my best regards

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Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the c...

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I purchased ab9071 and ab9072 as they are noted on the Abcam website to be useful as a matched pair for sELISA. However the anti-mouse HRP secondary is binding to the capture antibody as well as the mouse detection antibody, producing a very high signa...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacements for one vial of ab63913 and one vial of ab16284 with the order number 1147505.
...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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