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Rabbit IgG, whole molecule
Our Abpromise guarantee covers the use of ab6802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/3000.|
|Immunomicroscopy||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.|
|ICC/IF||1/500 - 1/2000.|
|IHC-P||1/500 - 1/2000.|
|IHC-Fr||1/500 - 1/2000.|
IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6802, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
This image is courtesy of an anonymous Abreview.
This image is courtesy of an Abreview submitted by Min young Kim