Overview

  • Product nameDonkey Anti-Rabbit IgG H&L (HRP)
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
  • Target speciesRabbit
  • Tested applicationsWB, ELISA, Immunomicroscopy, Dot Blot, ICC/IF, IHC-P, IHC-Frmore details
  • Immunogen

    Rabbit IgG, whole molecule

  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesHorseradish Peroxidase (HRP).
  • Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads
  • Clonality Polyclonal
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/3000.
ELISA 1/160000.
Immunomicroscopy Use at an assay dependent concentration.
Dot Blot Use at an assay dependent concentration.
ICC/IF 1/500 - 1/2000.
IHC-P 1/500 - 1/2000.
IHC-Fr 1/500 - 1/2000.

Donkey Anti-Rabbit IgG H&L (HRP) images

  • IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6802, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre


  • developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview.

    See Abreview


  • developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Min young Kim

    See Abreview

References for Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)

This product has been referenced in:
  • Son ED  et al. Staphylococcus aureus inhibits terminal differentiation of normal human keratinocytes by stimulating interleukin-6 secretion. J Dermatol Sci 74:64-71 (2014). Read more (PubMed: 24398033) »
  • Larocque K  et al. Novel analogue of colchicine induces selective pro-death autophagy and necrosis in human cancer cells. PLoS One 9:e87064 (2014). WB . Read more (PubMed: 24466327) »

See all 10 Publications for this product

Product Wall

Thank you for contacting us.

we tested catalog ab126709in IP, IHC, ICC, and Flow cytometry and it is tested negative. For this reason, we only recommending the product for western blot.

I hope this information is helpful to you. Pleas...

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The ab22696 could be used at 1/10 - 1/100 dilution.
ab54615 at 5-10ug/ml
ab103724 at 5-10ug/ml
ab126709 at 1/500-1/1000 dilution.

ab6820 and ab6802 at 1/500-1/2000 dilution.

I hope thi...

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Thank you for contacting us.

The secondary antibodies you mentioned might work for dot blots with the rabbit polyclonal Histone primaries, as they are guaranteed to work in Western blot which is quite similar to dot blot. However, we have not...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"