Overview

  • Product nameDonkey Anti-Rabbit IgG H&L (HRP)See all IgG secondary antibodies ...
  • Target speciesRabbit
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • Tested applicationsICC, IHC-P, ELISA, WB more details
  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notesMolar enzyme/ antibody protein ratio is 4:1
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000.
WB 1/5000 - 1/50000. Colorimetric: Use 1/5000-1/30000, Chemiluminescent: Use 1/10000-1/50000

Donkey Anti-Rabbit IgG H&L (HRP) images

  • Donkey polclonal secondary antibody to rabbit IgG-H&L (HRP) (ab97064) was used in a dilution of 1:5000. The first antibody was Smad4 antibody [EP618Y] (ab40759). The gel was a 4%-12% Bis-Tris Gel with MOPS Buffer. The first band is 30µg nuclear extract of a EBV-transformed B-lymphoblastoid cell line, the second band is 50µg of whole protein extract of the same EBV transformed B-LCL and the third lane is the same than in the second one but only 30µg. In this combination the secondary antibody works excellent. There is only the predicted band of 60 kDa, no addtional bands!

    This image is courtesy of Mr Daniel Meier

References for Donkey Anti-Rabbit IgG H&L (HRP) (ab97064)

This product has been referenced in:
  • Wang Y  et al. Drug target prediction based on the herbs components: the study on the multitargets pharmacological mechanism of qishenkeli acting on the coronary heart disease. Evid Based Complement Alternat Med 2012:698531 (2012). WB . Read more (PubMed: 22474514) »
  • Albani MC  et al. PEP1 of Arabis alpina is encoded by two overlapping genes that contribute to natural genetic variation in perennial flowering. PLoS Genet 8:e1003130 (2012). WB ; Arabidopsis thaliana . Read more (PubMed: 23284298) »

See all 2 Publications for this product

Product Wall

We have not specifically tested this Anti-CD9 antibody [EPR2949] (ab92726) as a marker of urinary exosomes but I can confirm that it can be used in western blot on mouse, rat, and human samples. Additionally, I have done a Google search and found that ...

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Merci beaucoup pour votre émail et votre intérêt pour nos produits.

Nous vous avons envoyé un devis pour le ab72422 contenant aussi l'anticorps secondaire ab97064. Le ab97064 est un anticorps contre les immunoglobul...

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The secondary antibodies you mentioned might work for dot blots with the rabbit polyclonal Histone primaries, as they are guaranteed to work in Western blot which is quite similar to dot blot. However, we have not...

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Thank you for your enquiry regarding ab97064. After reviewing the WB image and the details of the experiments, it could well be that the band at around 50 kDa represents the heavy chain of the immunoglobulin on the blot. The light chain can be se...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"