Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7083)

Overview

  • Product nameDonkey Anti-Rabbit IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Target speciesRabbit
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Frmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • Immunogen

    Full length native Rabbit IgG (purified).

  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads
  • Conjugation notesHorseradish Peroxidase (HRP)
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/3000.
IHC-P Use at an assay dependent dilution.
WB 1/1000 - 1/3000.
ELISA 1/7500 - 1/30000.
Immunomicroscopy Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

References for Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7083)

This product has been referenced in:
  • Faulkner S  et al. A comparison of the bovine uterine and plasma proteome using iTRAQ proteomics. Proteomics 12:2014-23 (2012). Read more (PubMed: 22623423) »
  • Kashino SS  et al. Initiation of acquired immunity in the lungs of mice lacking lymph nodes after infection with aerosolized Mycobacterium tuberculosis. Am J Pathol 176:198-204 (2010). IHC-P . Read more (PubMed: 20008132) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us again and sending this new information.

The WB image looks very convincing and proofs that the antibody is actually picking up its target as it should.

Regarding the double band in the pup sample it may be ...

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Thank you for your reply.

Unfortunately, it is quite hard to get some information if this protein expressed in the rat hippocampus. However, I checked the SwissProt site for more information regardingthis for the human protein, and it looks li...

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Thank you for resending the image. Based on your description of the upper band as being ˜ 25kD it seems this could be the correct band, as the size can vary a few kD just based on the gel, buffer, etc. Adding a negative control could help ensure that t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"