Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97085)

Overview

  • Product name
    Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed
  • Target species
    Rabbit
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
  • Tested applications
    Suitable for: ICC, IHC-P, WB, ELISAmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Human, Mouse, Pig, Rat more details
  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
WB 1/5000 - 1/50000. (Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000)
ELISA 1/10000 - 1/100000. (Primary ELISA)

Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed images

  • ab134047 staining VCAM1 in mouse aorta tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with methanol and blocked with 10% normal donkey serum for 2 hours at room temperature. Samples were incubated with primary antibody (1/200 in PBS + 0.1% Triton + 2% normal donkey serum) for 12 hours at 4°C. A HRP-conjugated donkey anti-rabbit IgG H&L (ab97085) (1/1000) was used as the secondary antibody

    See Abreview

References for Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97085)

ab97085 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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