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Product name Donkey Anti-Rabbit IgG H&L (TRITC) preadsorbed See all IgG secondary antibodies
Target species Rabbit
Tested applications Suitable for:
ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA more details
Minimal cross-reactivity Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep
To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
Full length native Rabbit IgG (purified).
Conjugation TRITC. Ex: 547nm, Em: 572nm Properties
Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Concentration information loading...
Purity IgG fraction
Purification notes Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons)
Absorption Wavelength: 550 nm
Emission Wavelength: 570 nm
Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG
Conjugation notes This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes Flow Cyt: Use at an assay dependant concentration.
IHC-P: Use at an assay dependant concentration.
IHC-Fr: Use at an assay dependant concentration.
IM: Use at an assay dependant concentration.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Donkey Anti-Rabbit IgG H&L (TRITC) preadsorbed images
Immunocytochemistry/ Immunofluorescence - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (TRITC ), pre-adsorbed (ab7080)
Ab7080 was used at dilution 1/200 with the primary antibody ab2900 in ICC/IF. See the review on ab2900.
References for Donkey Anti-Rabbit IgG H&L (TRITC) preadsorbed (ab7080)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"