Donkey Anti-Rabbit IgG H&L (TRITC) preadsorbed (ab7080)

Overview

  • Product name
    Donkey Anti-Rabbit IgG H&L (TRITC) preadsorbed
    See all IgG secondary antibodies
  • Target species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • Immunogen

    Full length native Rabbit IgG (purified).

  • Conjugation
    TRITC. Ex: 547nm, Em: 572nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG
  • Conjugation notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Immunomicroscopy
Flow Cyt
IHC-P
IHC-Fr
ELISA
  • Application notes
    Flow Cyt: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    IM: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Donkey Anti-Rabbit IgG H&L (TRITC) preadsorbed images

    • Ab7080 was used at dilution 1/200 with the primary antibody ab2900 in ICC/IF. See the review on ab2900.

    References for Donkey Anti-Rabbit IgG H&L (TRITC) preadsorbed (ab7080)

    ab7080 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab7080.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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