Donkey Anti-Rat IgG H&L (Alexa Fluor® 568) preadsorbed (ab175475)

Overview

  • Product name
    Donkey Anti-Rat IgG H&L (Alexa Fluor® 568) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Donkey
  • Target species
    Rat
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISAmore details
  • Minimal
    cross-reactivity

    Human, Mouse more details
  • Conjugation
    Alexa Fluor® 568. Ex: 578nm, Em: 603nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 30% Glycerol, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
Flow Cyt 1/2000.
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Images

  • ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 1µg/ml) overnight at +4°C. The secondary antibody (yellow) was ab175475 Alexa Fluor® 568 donkey anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Oh YS  et al. ESR1 inhibits hCG-induced steroidogenesis and proliferation of progenitor Leydig cells in mice. Sci Rep 7:43459 (2017). IF . Read more (PubMed: 28266530) »
  • Kasioulis I  et al. Inter-dependent apical microtubule and actin dynamics orchestrate centrosome retention and neuronal delamination. Elife 6:N/A (2017). Read more (PubMed: 29058679) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (PFA fixed)
Great!!
Username

Abcam user community

Verified customer

Submitted Jan 25 2016

Application
Immunohistochemistry (PFA fixed)
This Secondary antibody works very well (1:250)
Username

Mr. Musaad Alshammari

Verified customer

Submitted Jan 19 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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