Overview

  • Product nameDonkey Anti-Rat IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Rat IgG - H&L (HRP), pre-adsorbed
  • Target speciesRat
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species.
  • Tested applicationsSuitable for: IHC-P, ICC, ELISA, WBmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, Sheep more details
  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross absorbed using bovine, chicken, goat, horse, human, mouse, rabbit and sheep immunosorbents to remove cross reactivity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Fragments were conjugated to HRP.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/5000.
ICC 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/5000 - 1/50000. Colorimetric: 1/5000 - 1/30000. Chemiluminescent: 1/10000 - 1/50000.

References for Donkey Anti-Rat IgG H&L (HRP) preadsorbed (ab102265)

This product has been referenced in:
  • Müller N  et al. B Lymphocyte Stimulator (BLyS) Is Expressed in Human Adipocytes In Vivo and Is Related to Obesity but Not to Insulin Resistance. PLoS One 9:e94282 (2014). WB ; Human . Read more (PubMed: 24728308) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"