Donkey anti-Rat IgG H&L (PE) secondary antibody (ab7008)

Overview

  • Product nameDonkey anti-Rat IgG H&L (PE) secondary antibody
    See all IgG secondary antibodies
  • Target speciesRat
  • Tested applicationsSuitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Frmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheep more details
  • Immunogen

    Rat IgG whole molecule

  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPrepared from monospecific antiserum by immunoaffinity chromatography using rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • Conjugation notesR-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • ClonalityPolyclonal
  • General notes


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
Immunomicroscopy 1/50 - 1/200.
Flow Cyt 1/100 - 1/250.
The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
IHC-P 1/50 - 1/200.
IHC-Fr 1/50 - 1/200.

Donkey anti-Rat IgG H&L (PE) secondary antibody images

  • Immunocytochemsity/Immunofluorescence analysis of lung tissue using Donkey anti-rabbit IgG H&L (PE) secondary antibody (ab7008).

  • Ab7008 was used with the primary antibody ab19765 in Flow Cyt. See Abreview on ab19765.

References for Donkey anti-Rat IgG H&L (PE) secondary antibody (ab7008)

ab7008 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Immunocytochemistry/ Immunofluorescence
IF stain in lung tissue and using secondary antibody.
this antibody is good because it is very specific and has good color.
Username

Abcam user community

Verified customer

Submitted Dec 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"