Purification notesThis product has been immuno-affinity purified and immuno cross-absorbed to reduce non-specific reactions.
The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353.
This product is an immunogold reagent built around colloidal gold particles of 6nm. The paritcle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 6nm particle size conjugate is less than 12 %.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesElectron Microscopy: Use at an assay dependent dilution. The method of production of this product allows the small particle size of 6nm to be used instead of 5nm with the same sensitivity but increased visibility in the standard transmission electron microscope (See also van Bergen en Henegouwen & Leunissen, Histochemistry, 85, (1986), 81.)
Light Microscopy: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
References for Donkey Anti-Sheep IgG H&L (6nm Gold) (ab39602)
This product has been referenced in:
Hollinshead M et al. Endocytic tubules regulated by Rab GTPases 5 and 11 are used for envelopment of herpes simplex virus. EMBO J31:4204-20 (2012).
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