Donkey Anti-Sheep IgG H&L (Biotin) (ab6899)
Key features and details
- Donkey Anti-Sheep IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Immunomicroscopy, Dot blot
Overview
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Product name
Donkey Anti-Sheep IgG H&L (Biotin)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Sheep -
Tested applications
Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Immunomicroscopy, Dot blotmore details -
Immunogen
Full length native Sheep IgG (purified).
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities -
Conjugation notes
Biotin/Protein Ratio: about 6 per IgG molecule -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/1000 - 1/5000.
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IHC-P |
1/1000 - 1/5000.
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IHC-Fr |
1/1000 - 1/5000.
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WB |
1/2000 - 1/10000.
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ELISA |
1/20000 - 1/100000.
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Immunomicroscopy |
Use at an assay dependent concentration.
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Dot blot |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
1/1000 - 1/5000. |
IHC-P
1/1000 - 1/5000. |
IHC-Fr
1/1000 - 1/5000. |
WB
1/2000 - 1/10000. |
ELISA
1/20000 - 1/100000. |
Immunomicroscopy
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (6)
ab6899 has been referenced in 6 publications.
- Greenfield SA et al. Characterization of a Bioactive Peptide T14 in the Human and Rodent Substantia Nigra: Implications for Neurodegenerative Disease. Int J Mol Sci 23:N/A (2022). PubMed: 36361905
- Recasens M et al. Astrocyte-targeted IL-10 production decreases proliferation and induces a downregulation of activated microglia/macrophages after PPT. Glia N/A:N/A (2018). PubMed: 30548340
- Davis EA et al. Long-term effect of parasympathetic or sympathetic denervation on intestinal epithelial cell proliferation and apoptosis. Exp Biol Med (Maywood) 242:1499-1507 (2017). PubMed: 28766984
- Blackmore K et al. LKB1-AMPK modulates nutrient-induced changes in the mode of division of intestinal epithelial crypt cells in mice. Exp Biol Med (Maywood) 242:1490-1498 (2017). PubMed: 28766983
- Petković F et al. Astrocyte-targeted production of interleukin-6 reduces astroglial and microglial activation in the cuprizone demyelination model: Implications for myelin clearance and oligodendrocyte maturation. Glia 64:2104-2119 (2016). PubMed: 27535761
- Wienecke J et al. Spinal cord injury enables aromatic L-amino acid decarboxylase cells to synthesize monoamines. J Neurosci 34:11984-2000 (2014). PubMed: 25186745