Overview

  • Product name
    Donkey Anti-Sheep IgG H&L (Biotin)
    See all IgG secondary antibodies
  • Target species
    Sheep
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Immunomicroscopy, Dot blotmore details
  • Immunogen

    Full length native Sheep IgG (purified).

  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Conjugation notes
    Biotin/Protein Ratio: about 6 per IgG molecule
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
IHC-Fr
WB
ELISA
Immunomicroscopy
Dot blot
  • Application notes
    Dot: Use at an assay dependant dilution.
    ELISA: 1/30000 - 1/130000.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    IM: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Donkey Anti-Sheep IgG H&L (Biotin) (ab6899)

    This product has been referenced in:
    • Wienecke J  et al. Spinal cord injury enables aromatic L-amino acid decarboxylase cells to synthesize monoamines. J Neurosci 34:11984-2000 (2014). Read more (PubMed: 25186745) »

    See 1 Publication for this product

    Product Wall

    Merci pour votre appel et votre patience.

    Je viens d'avoir la confirmation du laboratoire que effectivement le ratio de conjugaison de la biotine était de ˜6 moles de biotine par mole de IgG.

    Je suis désolée pou...

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    Thank you for your enquiry. We have yet to receive reviews from customers who have purchased and tested this antibody in WB or IHC. We also, don't have the same product with a fluorescent conjugate. To view a full list of our secondary antibodies, you ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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