Donkey Anti-Sheep IgG H&L (DyLight® 488) (ab96939)

Overview

  • Product nameDonkey Anti-Sheep IgG H&L (DyLight® 488)
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Sheep IgG - H&L (DyLight® 488)
  • Target speciesSheep
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Sheep IgG and with light chains common to other Sheep immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cytmore details
  • ConjugationDyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Donkey Anti-Sheep IgG H&L (DyLight® 488) images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Donkey Anti-Sheep IgG H&L (DyLight® 488) (ab96939)

ab96939 has not yet been referenced specifically in any publications.

Product Wall

According to my records, ab139923 was proving difficult to use in IHC and we were in contact in order to help resolve the issue.



Looking at our correspondence, it appears that we are awaiting protocol details in order to help us...

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We recommend using the filter used for FITC.

Thank you for contacting Abcam regarding ab96939.


I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check th...

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Thank you for your reply. My colleague is not in the office today and that is the reason I am writing to you.

I ma sorry there was a misunderstanding.

We do have information on the spectra of DyLight® fluorochromes on our homepage...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"