Donkey Anti-Sheep IgG H&L (DyLight® 550) (ab96940)

Overview

  • Product nameDonkey Anti-Sheep IgG H&L (DyLight® 550)
    See all IgG secondary antibodies
  • Target speciesSheep
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Sheep IgG and with light chains common to other Sheep immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cytmore details
  • ConjugationDyLight® 550. Ex: 562nm, Em: 576nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notesDyLight® 550 replaces DyLight® 549.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500. DyLight®550 replaces DyLight®549. The DyLight®550 excitation is 562nm and the emission is 576nm. DyLight®550 provides the same orange-to-red fluorescence and photostability as the DyLight®549 dye. Please refer to the vial in order to check whether the product you received is DyLight®549 or DyLight®550. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight550.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Donkey Anti-Sheep IgG H&L (DyLight® 550) images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Donkey Anti-Sheep IgG H&L (DyLight® 550) (ab96940)

ab96940 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"